Nitric Oxide and Mechanisms of Redox Signaling

Nitric oxide and mechanisms of redox signaling.

Regulation of signal transduction and gene expression is a multifaceted process involving ligands, receptors, and second messengers that trigger cascades of protein kinases and phosphatases and propagate the signal to the nucleus to alter gene expression. Reduction-oxidation (redox)-based regulatory pathways provide additional means of gating signal transduction, and redox-based regulation of g...

Redox couples of inducible nitric oxide synthase.

We report direct electrochemistry of the iNOS heme domain in a DDAB film on the surface of a basal plane graphite electrode. Cyclic voltammetry reveals FeIII/II and FeII/I couples at -191 and -1049 mV (vs Ag/AgCl). Imidazole and carbon monoxide in solution shift the FeIII/II potential by +20 and +62 mV, while the addition of dioxygen results in large catalytic waves at the onset of FeIII reduct...

Signaling and Redox Regulation by Nitric Oxide, Superoxide and Carbon Monoxide

Hemoglobin-mediated nitric oxide signaling.

The rate that hemoglobin reacts with nitric oxide (NO) is limited by how fast NO can diffuse into the heme pocket. The reaction is as fast as any ligand/protein reaction can be and the result, when hemoglobin is in its oxygenated form, is formation of nitrate in what is known as the dioxygenation reaction. As nitrate, at the concentrations made through the dioxygenation reaction, is biologicall...

Nitric oxide signaling in plants

Nitric oxide (NO) is now seen as a vitally important molecule in many biological systems. Once it was identified in mammalian systems in 1987 (Palmer et al., 1987) it was only a matter of time before researchers hunted for its presence in plants, with the first such reports being published in 1998 (Delledonne et al., 1998; Durner et al., 1998). Now 15 years later the interest in NO and its role...